The kinetics of solubilization of ethyl hexanoate into an aqueous phase was investigated in the presence of various additives (NaCl, ethanol, and sodium hexanoate). The salting-out effect of NaCl and the solvent effect observed in the presence of ethanol were quantified. Solubilization by sodium hexanoate shows a dramatic enhancement when the critical micellar concentration is exceeded. This effect was interpreted by the formation of mixed oil/surfactant aggregates. The kinetic rate constants of solubilization of ethyl hexanoate into the water phase were determined in the presence of sodium hexanoate using a model including salting-in, salting-out, and aggregation effects. When NaCl was replaced by NaOH, ethyl hexanoate was hydrolyzed to sodium hexanoate and ethanol. The kinetics of this reaction was recorded in the same geometrical setup as for previous solubilization experiments. The solubilization kinetic model was able to describe the hydrolysis reaction by just adding a rapid bulk hydrolysis step. The model quite accurately predicts the autocatalytic effect of sodium hexanoate formation and the overall reaction time. This result clearly shows the crucial role of solubilization processes on this biphasic hydrolysis reaction.