한외여과를 이용한 α-Amylase의 분리특성

김현수; 유영제

HWAHAK KONGHAK, Vol.28, No.3, 313-319, June, 1990

Export Citation

한외여과를 이용한 α-Amylase의 분리특성

Separation of α-Amylase using Cross-Flow Ultrafiltration

김현수, 유영제

초록

십자흐름 한외여과장치를 이용하여 α-amylase를 분리하는 경우 조업조건이 분리효율에 미치는 영향을 실험적으로 관찰하였다. 여과압력이 높을수록 그리고 유속이 낮을수록 분리효율은 증가하였다. 또한 여과압력이 높고 유속이 낮으며, α-amylase의 농도가 낮은 경우 효소활성이 심하게 저하되었다. 여과유속을 예측하기 위하여 모델식을 이용하여 실제 실험치와 비교한 결과 α-amylase의 농도가 낮은 경우 예측치와 실험치가 잘 일치하였다.

The effects of operating conditions on the separation efficiency of α-amylase employing cross-flow ul-trafiltration unit were investigated. High transmembrane pressure and low rate gave a higher separation ef-ficiency. Severe deactivation of the enzyme during cross-flow ultrafiltration was observed when the transmembrane pressure was high and both flow rate and the enzyme concentration were low. The permeate flow rate could be well predicted by using related model equations, especially for the feed stream of low enzyme activities.

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[1] Strathmann H, Trends Biotechnol., 3, 112 (1985)

[2] Trettin DR, Doshi MR, Ind. Eng. Chem. Fundam., 19, 189 (1980)

[3] Vilker VL, Colton CK, Smith KA, AIChE J., 27, 4 (1981)

[4] Bodzek M, Kominsk D, Environ. Prot. Eng., 5, 3 (1979)

[5] Hong J, Lee CK, Annals N.Y. Academy Sci., 469, 131 (1986)

[6] Lloyd DR, Am. Chem. Soc. Symp., 269, 1 (1985)

[7] Mathiasson E, Sivik B, Desalination, 35, 59 (1980)

[8] Porter MC, Ind. Eng. Chem. Process Des. Dev., 11, 234 (1972)

[9] Lopez-Leiva M, Polym. Sci. Technol., 13, 269 (1980)

[10] Blatt WF, Dravid AS, Michaels NL, "Membrane Science and Technology," (J.E. Flinn, ed.) Plenum, New York, pp. 47-97 (1970)

[11] Gekas V, Hallstroem B, J. Membr. Sci., 30, 153 (1987)

[12] Schulz G, Ripperger S, J. Membr. Sci., 40, 173 (1989)

[13] Leveque MA, Ann. Mines., 14, 1 (1928)

[14] Lafaille JP, Sanchez V, Machenc J, Int. Chem. Eng., 27, 258 (1987)