Three different biomasses, namely, sericulture waste, Populus deltoides, and Eupatorium adenophorum, were studied for biogas production. It was found that these wastes produced biogas in reasonably good quantity of gas (259L/kg of total solid in sericulture waste to 519L/kg of total solid in E. adenophorum). The substrates were subjected to anaerobic digestion directly, without any pretreatment, and a batch with biological treatment in triplicate set. Among the two sets, the biologically pretreated digesters gave higher yield of biogas. Chemical analysis for nitrogen content, cellulose, hemicellulose, and lignin content before and after digestion was carried out.