Biotechnology Letters, Vol.21, No.4, 355-359, 1999
Selective enzymatic preparation of vinyl sugar esters using DMSO as a denaturing co-solvent
The protease-catalyzed transesterifications between hexoses and divinyladipate were examined. In dimethylformamide hexoses such as D-glucose, D-mannose, D-galactose and alpha-methyl D-galactoside were esterified with divinyladipate by alkaline protease from Streptomyces sp. to give corresponding 6-O-vinyl adipoyl sugars. When the denaturing cosolvent, DMSO, was added to the solvent, galactose was selectively esterified at only the C-2 position.
Keywords:ORGANIC-SOLVENTS;DIMETHYLFORMAMIDE;PROTEASE;BIOSURFACTANTS;ACYLATION;ALCOHOLS;GLUCOSE;MEDIA