Structured triacylglycerols resembling human milk fat, that contain palmitoyl (16:0) moieties predominantly at the sn-2-position of the glycerol backbone and oleoyl (18:1) and linoleoyl (18:2) moieties at the sn-1,3-positions, have been prepared by regiospecific transesterification of tripalmitin with fatty acids of low-erucic rapeseed oil using a plant lipase - papaya latex - and for comparison Lipozyme, as biocatalyst.