Biotechnology Letters, Vol.20, No.5, 463-468, 1998
Light-enhanced target gene expression in tobacco BY-2 by the combination of overexpressed phytochrome and rbcS3A promotor
Enhanced expression of the rbcS3A promoter was achieved by overproducing phytochrome in BY-2 tobacco cells. The transformed calls contained the oat phytochrome A gene fused the 35S promoter of Cauliflower Mosaic Virus (CaMV) and the gus gene to the rbcS3A promoter. The transformed cells exhibited 1.5- to -2.8 fold enhanced the beta-glucuronidase (GUS) activity when subjected to light.