NAD(+) Binding by Yeast Alcohol-Dehydrogenase in the Presence of Pyrazole and a New Method for the Determination of the Enzyme Active-Site Concentration

Trivic S; Zeremski J; Leskovac V

Biotechnology Letters, Vol.19, No.8, 809-811, 1997

DOI10.1023/A:1018308830633 Export Citation

NAD(+) Binding by Yeast Alcohol-Dehydrogenase in the Presence of Pyrazole and a New Method for the Determination of the Enzyme Active-Site Concentration

Trivic S, Zeremski J, Leskovac V

The pyrazole method of Theorell and Yonetani (Biochem. Z., 338 : 573-553, 1963) has been adapted for the determination of the enzyme active site concentration in yeast alcohol dehydrogenase (EC 1.1.1.1). This method cannot be applied indiscriminately to other alcohol dehydrogenases without modification.