Biotechnology Letters, Vol.19, No.7, 623-627, 1997
Secretion of Invertase from Schwanniomyces-Occidentalis
Invertase synthesis in Schwanniomyces occidentalis is regulated by catabolite repression and is derepressed by raffinose and low concentrations of glucose. Efficiency of a carbon source in derepression of invertase is dependent upon the type of culture medium : either raffinose in a rich medium or a low concentration of glucose in a yeast minimal medium. The kinetics of derepression can be modulated by changing the carbon source. When cells are grown in a rich medium with 0.5% raffinose as the sole carbon source, Schwanniomyces occidentalis secretes 80 times more invertase than Saccharomyces cerevisiae grown in the same conditions, About 50% of the total amount of invertase produced by Schwanniomyces occidentalis is secreted in the extracellular medium in contrast to Saccharomyces cerevisiae where only 6 to 15% of the protein is secreted in the medium.
Keywords:SACCHAROMYCES-CEREVISIAE;ALPHA-AMYLASE;SEQUENCE-ANALYSIS;ADE2 GENE;YEAST;CLONING;EXPRESSION;TRANSFORMATION;PURIFICATION;CASTELLII