Biotechnology Letters, Vol.19, No.5, 475-478, 1997
Biochemical Synthesis of Chirally Pure RP Oligonucleotide Phosphorothioates
Oligonucleotide phosphorothioates have been synthesized using procaryotic DNA polymerase and oligonucleotide template/primer. The method facilitates the recovery of DNA polymerase and template/primer and is successful at the milligram scale.
Keywords:HUMAN-IMMUNODEFICIENCY-VIRUS;ANALOGS