A facultative anaerobic pure bacterial culture L-2 capable of growth on 12.5% (v/v) diluted digested spent wash supplemented with glucose (10 g/l) was isolated from an Indian distillery. It achieved 31% decolorization and 57% COD reduction after 7 days’ incubation. The advantages of using such a culture for digested spent wash bioremediation are apparent in providing a realistic approach for decreasing its pollution potential prior to disposal.