화학공학소재연구정보센터
Biotechnology Letters, Vol.18, No.12, 1435-1438, 1996
Production of Constitutive, Thermostable, Hyperactive Exo-Pectinase from Bacillus GK-8
A new alkalophilic Bacillus GK-8 overproduced three thermostable extracellular pectinases. The temperature optima was 60 degrees C for all the three enzymes which retained full activity for 2 h. They had pH optima 5.4, 7.0 and 10.4 and were designated as PT, PII and PIII respectively. The half life at 80 degrees C of PI, PII and PIII was 18 min., 12 min. and 12 min., respectively. The enzyme activity was stimulated by Mg2+, Ca2+, Zn2+, Co2+ and Mn2+ ions.