The characteristics of poly-beta-hydroxybutyrate (PHB) production from glucose or alcoholic distillery wastewater by isolated Actinobacillus sp. EL-9 were investigated. PHB production was not dependent on nutrients limitation in Actinobacillus sp. EL-9. The PHB accumulation of Actibobacillus sp. EL-9 followed a growth-associated type where the cell growth and PHB accumulation were carried out simultaneously. The Actinobacillus sp. EL-9 was shown to synthesize and accumulate PHB from alcoholic distillery wastewater during growth, The best growth and PHB production were obtained with enzyme-hydrolyzed alcoholic distillery wastewater.