The effect of different concentrations of carbohydrates, nitrogen, sulphate, plant growth regulators and elicitors on growth and thiophene accumulation by transformed root cultures of Tagetes laxa (Cabrera) was studied. The combinations of sucrose (30 g/l), nitrogen (60 mM), sulphate (150 mM) and the ratio N-ox:N-red 2.1 are the most appropriate combination to support growth and thiophene accumulation, which was increased by 90% when the cultures were elicited with homogenate of Sclerotinia Sclerottiorum. The plant growth regulators used produced dedifferentiation with a decrease in thiophene biosynthesis.