An extracellular alkaline lipase was evaluated from Pseudontonas pseudoalcaligenes F-111, which was isolated from soil using a rhodamine B agar plate with Na2CO3. For enzyme production, olive oil, soymeal, triton X-100 and sodium ion were found to be essential The addition of triton X-100 increased the alkaline lipase by 50- fold. The effect of additives as well as the development of suitable culture conditions for lipase production is discussed.