The minicell producing strain Bacillus subtilis IA292 was transformed with plasmids encoding the Bacillus enzymes beta-glucanase, alpha-amylase and neutral protease. Purified minicells were shown to be free of detectable proteolytic activity. Minicells containing plasmids were found to synthesise an three enzymes internally, but evidence of secretion was only observed in the unique case of neutral protease secretion by minicells prepared from cultures grown in BHI medium.