The regenerated Antheraea pernyi Silk fibroin films prepared from calcium nitrate solution were treated with 80% aqueous methanol. The thermal and dynamic thermomechanical behaviors of the films were investigated through TGA, DSC and DMTA. IR spectroscopy was used for the conformational changes with methanol treatments. The treatment time of aqueous methanol highly influenced the thermal behavior of regenerated films. As the methanol treatment time increased up to 60 min, the thermal decomposition behavior did not differ significantly compared with untreated Trims while the endo/exo transition was gradually decreased, with the exotherm finally disappearing. The dynamic mechanical thermal behavior was also affected by the treatment rime of aqueous methanol. The onset temperature of storage modulus drop or damping peak shifted to a higher temperature due to the increase in crystallinity induced by the methanol treatment. The thermal transition was strongly dependent on the formation of beta-sheet crystal conformation of the regenerated films upon methanol treatment.