Biotechnology Letters, Vol.23, No.3, 215-219, 2001
Biotransformation of oleic acid into (E)-10-hydroxy-8-octadecenoic acid and (E)-7,10-dihydroxy-8-octadecenoic acid by Pseudomonas sp 42A2 in an immobilized system
Oleic acid was transformed into 10-hydroxy-8E-octadecenoic acid and 7,10-dihydroxy-8E-octadecenoic acid using Pseudomonas sp. 42A2 NCIMB 40045 in different immobilised supports. Celite R633 gave highest conversions in 48 h: the cellular yield (Y-p/x) g products g(-1) of cellular protein in the immobilised culture was 5 compared with the free-cell culture Y-p/x of 3.8. Conversion of oleic acid in the immobilised cell culture was 50% of the carbon source supplied.