Biotechnology Letters, Vol.23, No.2, 103-109, 2001
Manganese peroxidase gene of the perennial mushroom Elfvingia applanata: cloning and evaluation of its relationship with lignin degradation
A gene encoding a manganese-dependent peroxidase (MnP) of the oriental mushroom Elfvingia applanata has been cloned into lambda gt10 and characterized. This gene, Ea.mnp1, consists of a 1,095 bp open reading frame coding for 364 amino acid residues. Northern blot analysis revealed that transcription of the Ea.mnp1 gene was elevated by an increase in Mn2+ to 200 mM or 0.1 g 2,5-xylidine l(-1). The Ea.mnp1 mRNA transcript levels changed in parallel with the observed changes in activity of MnP in E. applanata.