Thin Solid Films, Vol.284-285, 18-23, 1996
Use of Planar Lipid Monolayers for 2D Crystallization of Proteins and Simple Analysis of Specific Lipid-Peptide Interactions
Planar lipid monolayers provide a model system for analysing molecular mechanisms of interactions between proteins and cell membranes. Small cell lung carcinoma (SCLC) constitutes 25% of the total deaths from lung cancer. Insertion of the substance P(SP) analogues [DArg(1), Dphe(5), DTrp(7,9), Leu(11)] SP and [Arg(6), DTrp(7,9)], MePhe(8)] SP (6-11), into phospholipid monolayers correlated with their biological activity in regulating growth in SCLC cell lines. The transmembrane helical peptides of the proto-oncogenic and oncogenic forms of the neu protein showed similar lipophilicity, suggesting receptor stabilisation without conformational change. The lipid-binding proteins annexin VI and V interacted most strongly with phosphatidylethanolamine and phosphatidylserine respectively and formed 2D crystals of trimers on phospholipid monolayers.
Keywords:TYROSINE KINASE-ACTIVITY;GROWTH-FACTOR RECEPTOR;MULTICATALYTIC PROTEINASE;NEU ONCOGENE;TRANSMEMBRANE DOMAIN;LIPOCORTIN-I;SUBSTANCE-P;PHOSPHOLIPASE-A2;UTEROGLOBIN;PROTEASOME