The metabolic inhibition of the three series of SG, O-VG and 2,4-L totaling eleven kinds of Schiff base complexes and two kind of corresponding metal compounds on Aerobacter aerogenes and Staphylococcus sureus has been studied microcalorimetrically. The studies reveal how, with reaction of different complexes, the difference in the metabolic power-time curves of the two kinds of bacteria metabolism. According to the power-time curves, the multiplication rate constant, generation time and inhibitory ratio of bacterial growth have been calculated, characterizating quantitatively the inhibition of Schiff base-metal complexes on bacteria metabolism. The relationship between the bacterial growth rate constant k and Schiff base-metal complex concentration c is thereby established, and on the basis of the quantity of half-inhibitory C-1/2 of Schiff base-metal complexes, the relationship between the structure of Schiff base-metal complexes and its antibacterial activity has been further studied by comparing the half-inhibitory quantity. A discussion of the cases of Schiff base-metal complexes of the same ligand at different metal ions, and of the same ions but different ligand reacting with different bacteria, has also been made, respectively, in comparative depth. It is found that, as for different bacteria, the same complex has different antibiotic activities, reflecting the difference between different kinds of bacteria; the inhibition of the same kind of bacteria varies with different complexes, showing the difference in the structure of the complexes, and thus providing useful information for the synthetic designing and the reasonable clinical administration of medicine of Schiff base drugs.