In a biological system, water can be bound to the matrix with different energies and thus, when the latter is heated, be lost at subsequent stages according to the activation energies required to obtain the release of the different types of water. In this sense, particularly when combined with ancillary systems such as FT-IR spectroscopy, thermoanalytical techniques allow us to distinguish all the different types of water contained in a biological system. Using this approach, the water in the cerebellum of mice treated by subdural inoculation of ouabain (G-strophanthin) was studied. At the same time, the activity of Na,K-ATPase was followed in treated and untreated mice cerebella by means of calorimetry. The activity decreases as a function of the time elapsing between inoculation and the animal’s death. Experimental data are discussed in terms of inhibition of Na,K-ATPase and in terms of the modified water-matrix interaction, consequent to the changed mechanism of the diffusion transport deriving from the gradient in Na-ion concentration between the inside and outside of the cell wall.