Order-disorder transition of the Li vacancies in an intermetallic compound beta-LiGa has been presented based on the electrical and heat capacity measurements. Anomalous heat capacity has been observed at T-c = 233 K, with excess enthalpy of 72.5 J/mol. Abrupt change in the electrical resistivity has been observed at the same temperature of T-c. The observed anomalous behavior was compared with that of beta-LiAI in connection with the defect structure near the Li deficient beta-phase.