A membrane was prepared to facilitate the transport of histidine by impregnating a microfiltration membrane with a chemically activated, polymer hydrogel. The rate of histidine diffusion through the membrane was measured with time; mass transfer coefficients and effective diffusion coefficients were determined for intermediate stages of sequential modification. Both supported and nonsupported hydrogel transport properties were compared and evaluated. Results indicated that the supported membrane was mechanically stable as compared to the unsupported membrane, and more significantly, that the addition of divalent copper facilitated the transport of histidine.