Absence of P350 Subunit of DNA-Activated Protein-Kinase from a Radiosensitive Human Cell-Line

Leesmiller SP; Godbout R; Chan DW; Weinfeld M; Day RS; Barron GM; Allalunisturner J

Science, Vol.267, No.5201, 1183-1185, 1995

DOI10.1126/science.7855602 Export Citation

Absence of P350 Subunit of DNA-Activated Protein-Kinase from a Radiosensitive Human Cell-Line

Leesmiller SP, Godbout R, Chan DW, Weinfeld M, Day RS, Barron GM, Allalunisturner J

The radiosensitive rodent mutant cell line xrs-5 is defective in DNA double-strand break repair and lacks the Ku component of the DNA-activated protein kinase, DNA-PK. Here radiosensitive human cell lines were analyzed for DNA-PK activity and for the presence of related proteins. The radiosensitive human malignant glioma M059J cell line was found to be defective in DNA double-strand break repair, but fails to express the p350 subunit of DNA-PK. These results suggest that DNA-PK kinase activity is involved in DNA double-strand break repair.

Keywords:TERMINAL TRANSACTIVATION DOMAIN;RNA POLYMERASE-II;KU-PROTEIN;HUMAN CHROMOSOME-8;BINDING PROTEIN;SCID MUTATION;BREAK REPAIR;MICE;PHOSPHORYLATES;RADIATION