Rapid and novel deprotection conditions have been developed for the cleavage of oligodeoxyribonucleotides from cis-diol group based universal polymer supports and simultaneous removal of protecting groups from exocyclic amino functionalities of nucleic bases and internucleotide phosphates. The fully deprotected oligodeoxyribonucleotides were compared with the corresponding oligomers prepared and deblocked using standard protocols. They were found to be identical with respect to their retention times on HPLC and biological activity.