A series of polyacrylamide gel derivatives has been prepared by transamidation of crosslinked polyacrylamide polymer with various diamine of different structures such as ethylenediamine, hydrazine hydrate, jeffamine 148(H2NCH2CH2OCH2 CH2OCH2CH2NH2), bis(3-aminopropyl)poly(tetrahydrofuran) 750 and bis(3-aminopropyl)polytetrahydrofuran) 1100 (BAPPTHF-1100). The crosslinked polyacrylamide was prepared by using the free-radical polymerization technique of the acrylamide monomer. The transaminated polyacrylamide derivatives were acylated with 2,4-dichlorophenoxyacetyl chloride in DMF using triethylamine as acid acceptor. The amine contents of the aminated gel derivatives were estimated and the swelling characteristics of the gel derivatives were also estimated. The release rates of the 2,4-dichlorophenoxyacetic acid (2,4-D) were determined in different pH media by UV-spectrophotometric analyses. The release rate was lower in pH 4. The effect of the length of the pendant side-chain spacers on the release rates have been studied.