Process Biochemistry, Vol.34, No.6, 731-735, 1999
Effect of apple pomace-based Trichoderma inoculants on seedling vigour in pea (Pisum sativum) germinated in potting soil
Three Trichoderma inoculants were produced by growing T. viride, T. harzianum and T, pseudokoningii on apple pomace-based medium at 20 degrees C for 7 days. The Trichoderma inoculants were mixed with potting soil at a ratio of 1:5 (v/v) and the mixture was used for germination tests of pea (Pisum sativum) seeds. When T. viride, T, harzianum and T. pseudokoningii inoculants were applied to the soil, the germination rate of pea seeds increased by 20, 40 and 15%, respectively, compared to the potting soil control. After 5 days the average plant height increased by 52, 67 and 48%, respectively, while the average fresh weight of seedlings increased by 26, 63 and 25%, respectively. The average total phenolic content of pea seedlings increased by 14, 18, and 12% respectively. Among those three Trichoderma inoculants, however, only T. harzianum inoculant increased plant height and fresh weight significantly, while both T. harzianum and T. viride inoculants increased phenolic content of seedlings significantly. The apple pomace control increased the germination rate, plant height and fresh weight slightly, but did not increase the phenolic content of pea seedlings. These results suggest that Trichoderma species grown on apple pomace-based medium can enhance seed performance of peas according to some criteria of seedling vigour and thus have potential for use as seed treatment.
Keywords:SHRUNKEN-2 SWEET CORN;BIOLOGICAL-CONTROL;ALGINATE PELLETS;AZOSPIRILLUM;DISINFECTION;GLIOCLADIUM;GROWTH;FUNGI