Process Biochemistry, Vol.34, No.1, 49-53, 1999
Clarification of xanthan gum with extracellular enzymes secreted by Trichoderma koningii
Xanthomonas campestris cells present in xanthan fermentation broth were lysed by enzymes secreted by Trichoderma koningii. Protease which appeared responsible for cell lysis was produced by the fungus in solid-state fermentation of wheat bran optimally at pH 7 and 30 degrees C in 72 h. The culture filtrate, having protease in addition to other extracellular enzymes, was used as such to clarify xanthan solution and found to lyse the cells optimally at pH 7 and 50 degrees C. The maximum transparency of xanthan solution achieved was 63%.