Process Biochemistry, Vol.32, No.7, 561-565, 1997
Cellulase Production by Trichoderma Sp Grown on Corn Fiber Substrate
Trichoderma sp. strain 414 was cultivated in Tanaka media based on corn fibre pulp from corn starch production to test the hemicellulase- and cellulase-inducing properties of the pulp when used as carbon source in nutrient dispersions. The pulp contained up to 70% hydrolysable matter. Cellulase and xylanase production were controlled and their activities reached compatible levels. Preliminary analysis of bioreactor performance showed that the process required moderate mixing and aeration, pH 4.5, and a temperature range between 28 degrees C and 30 degrees C. Cellulase activity reached 3.34 U/cm(3), while xylanase activity achieved was 3.7 U/cm(3). The time-course of enzyme production was determined.
Keywords:ENZYMES