A sample of high-methoxyl citrus pectin was separated into its major fractions on diethylaminoethyl-cellulose columns using increasing concentrations of NaH2PO4 solution as the eluent. The fraction eluted with the lowest concentration of NaH2PO4 had the highest methoxyl content, and latter fractions, obtained with progressively increasing NaH2PO4 concentrations, showed progressively lower methoxyl values. The percentage esterification and equivalent weight also decreased in a similar manner. This decrease in methoxyl content from one fraction to the next was accompanied by an increase in setting times and jelly grades. A small decrease in molecular weight from one fraction to the next towards the low-ester range was observed and none of the fractions had a molecular weight as high as the original pectin.