The results of investigations on the effect of baker's yeast Saccharomyces cerevisiae suspension on the process of disintegration in a bead mill with a multi-disk impeller for various operating conditions of circulating packing in the working chamber are presented. The variable parameters were the concentration of microorganism suspension (0.05-0.20 g dry mass/ml), disk-to-disk distance (5-40 mm) and rotational speed of the impeller (1000-3500 rpm). Experiments were carried out for batch operation. Modelling of the process by describing its kinetics using the first-order differential equation was confirmed to be adequate. Hypothetical mechanisms of yeast cell disintegration in the mill are presented.