The properties of carriers made of acrylonitrile (AN) or butyl acrylate (BA) and crosslinked by trimethylolpropane trimethacrylate monomer (TMPMA) or trimethylolpropane triacrylate monomer (TMPA) are discussed. The extent of aminolysis of triacrylate copolymers depends on the comonomer composition. The carriers crosslinked with TMPMA have greater porosity and larger mean pore size than carriers that were crosslinked by TMPA. The enzyme activity on carriers that were obtained by ester group aminolysis in BA copolymers is higher than on carriers made of AN. It is found that TMPMA/BA carriers are the best for immobilization of peniccilin acylase.