The renaturation level of gelatin films was determined by calorimetry and polarimetry. Good agreement was observed for films made with conventional gelatins. A significant discrepancy was found on desiccated films (4% water), on films where KSCN had been added, and on films made with hydrolysed, chemically modified, or crosslinked gelatins. These results are discussed in relationship to the two techniques used and to the renaturation mechanism in steps. The discrepancy was attributed to the fact that some gelatin strands were frozen in intermediate states.