A proteoglycolipidic interfacial film is obtained;from the spreading of two types of glycolipid vesicles associated with luciferase through adsorption or inclusion processes. The vesicles＇ disintegration is followed by recording the increase in surface pressure versus time. The interfacial film thus obtained is compressed and transferred onto substrates, and the luciferase activity is assayed through bioluminescence studies. It was shown that the luciferase is inserted in an active form in the transferred films. Its kinetic behavior depends on the surface pressure at which the interfacial film was transferred and on the type of enzyme association.