Catalase, ascorbate oxidase, glucose oxidase and uricase immobilized on oxirane acrylic particles were packed into a column. Each sample of substrate solution of volume G and substrate concentration C was injected into the inlet flow of the fixed buffer solution flowing through the column at the rate v and temperature T, and the dissolved oxygen concentration in the outlet flow was analyzed by a dissolved oxygen sensor. Sensor output was integrated with respect to time to obtain S. In each sample, stoichiometric relations of S were formed approximately in under about S = 5.5 mv . min.