Synthesis of Glutamate by Reductive Amination of 2-Oxoglutarate with the Combination of Hydrogenase and Glutamate-Dehydrogenase - Scientific Note

Hasumi F; Miyamoto Y; Okura I

Applied Biochemistry and Biotechnology, Vol.55, No.1, 1-4, 1995

Hasumi F, Miyamoto Y, Okura I

Glutamate synthesis by reductive amination of 2-oxoglutarate was performed by the combination of NADH regeneration system and glutamate dehydrogenase (GluDH). The conversion of 2-oxo-glutamate to glutamate was 98% after 3 h, and the turnover number of NAD(+) was 17.