Today, the importance of biocompatible nanocomposite films with suitable properties for potential applications in wound healing has been considered. In present study, nanocomposite films of gelatin/aldehyde-gellan gum/Naringenin-loaded Zein nanoparticle (GL/AG/NR@Z) were successfully produced with the solution casting method. NR@Z with different concentrations (1.5 and 3 wt%) loaded into the GL/AG films. Then, the morphological, structural, thermal, and physical properties of these films were investigated. The obtained findings illustrated that by addition of NR@Z content a denser and more porous structure with high swelling ratio (around 80%), high thermal stability, high tensile stress (54.33 ± 3.45 MPa), and high weight loss (around 70%) was observed. Cell viability assessment of the produced films displayed no cytotoxicity for NIH3T3 fibroblast cells. Furthermore, since NR is a polyphenolic compound, the produced GL/AG/NR@Z nanocomposite films showed antioxidant and antibacterial activity. Consequently, we demonstrated that nanocomposite films (GL/AG/NR@Z) could be a suitable scaffold for wound healing applications.