화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Applied Chemistry for Engineering, Vol.33, No.2, 195-201, April, 2022
DOI10.14478/ace.2022.1013  Export Citation
지황 추출물 첨가 chitosan 기반 기능성 바이오 소재 제조 및 응용
Preparation and Application of Rehmannia Glutinosa Extract Incorporated Functional Chitosan Based Biomaterials
이시연, 김경중, 김윤섭†, 윤순도†
  • 전남대학교 화공생명공학과
Si-Yeon Lee, Kyeong-Jung Kim, Youn-Sop Kim†, and Soon-Do Yoon†
  • Department of Chemical and Biomolecular Engineering, Chonnam National University, Yeosu 59626, Korea
E-mail:,
초록
본 연구에서는 지황 추출물[Rehmannia glutinosa extract (RE)]이 함유된 chitosan (CH) 기반 바이오 소재를 제조하고, 물리·화학적 특성, RE 방출 특성, 멜라닌 합성 억제 효과, 항산화 효과, elastase 억제 효과를 조사하였다. RE 함유 CH 기반 바이오 소재는 casting method와 UV 경화 공정을 통해 제조되었다. 제조된 바이오 소재의 표면 특성은 FE-SEM으 로 분석하였고, 물리적 특성은 인장강도, 신축률을 통하여 조사하였다. 경피 약물 전달 시스템을 적용하기 위해 36.5 °C에서 pH 4.5, pH 5.5, pH 6.5 용액에서와 인공피부를 이용해 제조된 바이오 소재에서 RE의 방출 특성을 조사하였으 며, 그 결과, pH 6.5에서 가소제를 첨가하지 않은 바이오 소재와 가소제[glycerol (GL)와 citric acid (CA)]를 첨가하여 제조한 RE 함유 바이오 소재에서 RE의 방출이 pH 4.5에서 보다 약 1.10배 빠르게 일어남을 알 수 있었다. 또한, 인공 피부에서 RE의 방출은 약 6 h 동안 지속적으로 방출됨을 확인하였다. Tyrosinase assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, elastase assay를 통해 기능성을 평가하였으며, 가소제로 CA를 첨가하고 RE가 함유된 바이오 소재에서 각각 45.12%의 tyrosinase 활성율, 89.40%의 DPPH 라디칼 소거능, 59.94%의 elastase 활성율을 나타내었다.
The main objective of this work is to prepare Rehmannia glutinosa extract (RE) incorporated functional chitosan (CH) based biomaterials and evaluate their physical properties, RE release properties, inhibitory effect of melanogenesis, and antioxidant and elastase inhibitory activities. RE incorporated CH based biomaterials were synthesized by a casting method and UV curing process. The surface and cross sections of prepared biomaterials were characterized by a field emission scanning electron microscope (FE-SEM). The physical properties such as tensile strength and elongation at break were also investigated. To apply the transdermal drug delivery system, RE release properties were examined with pH 4.5, 5.5, and 6.5 buffer solutions and artificial skin test at 36.5 °C. Results indicated that RE release of RE incorporated biomaterials with/without the addition of plasticizers [glycerol (GL) and citric acid (CA)] at pH 6.5 was about 1.10 times higher than that of at pH 4.5. In addition, results of the artificial skin test verified that RE was released constantly for 6 h. To verify the applicability of the prepared biomaterials, tyrosinase, 2,2-diphenyl-1-picrylhydrazyl (DPPH), and elastase assays were investigated. Results indicated that RE incorporated biomaterials added CA exhibited tyrosinase activation, DPPH radical scavenging activity rate, and elastase activation of 45.12, 89.40, and 59.94%, respectively.
Keywords:Rehmannia glutinosa extract;Transdermal drug delivery system;Tyrosinase inhibition;DPPH radical scavenging;activity;Elastase inhibition
  1. Foster LJR, Ho S, Hook J, Basuki M, Marçal H, Plos One, 10, e0135153 (2015)
  2. Subramanian K, Sadaiappan B, Aruni W, Kumarappan A, Thirunavukarasu R, Srinivasan GP, Bharathi S, Nainangu P, Sci. Rep. UK., 10, 1 (2020)
  3. Sun X, Yu D, Ying Z, Pan C, Wang N, Huang F, Ling J, Ouyang X, Pharmaceutics, 11, 584 (2019)
  4. Lai W, Hu C, Deng G, Lui K, Wang X, Tsoi T, Wang S, Wong W, Int. J. Pharm., 566, 101 (2019)
  5. Hafeez S, Islam A, Gull N, Ali A, Khan SM, Zia S, Anwar K, Khan SU, Jamil T, Int. J. Biol. Macromol., 114, 890 (2018)
  6. Frandsen JL, Ghandehari H, Chem. Soc. Rev., 41, 2696 (2012)
  7. Berkland C, Pollauf E, Pack DW, Kim K, J. Control. Release, 96, 101 (2004)
  8. Allen TM, Cullis PR, Science, 303, 1818 (2004)
  9. Marwah H, Garg T, Goyal AK, Rath G, Drug Deliv., 23, 564 (2016)
  10. Tak HY, Yun YH, Lee CM, Yoon SD, Carbohydr. Polym., 208, 261 (2019)
  11. Nair LS, Laurencin CT, Prog. Polym. Sci, 32, 762 (2007)
  12. Kim JK, Kim HJ, Chung JY, Lee JH, Young SB, Kim YH, Arch. Pharm. Res., 37, 60 (2014)
  13. Yun YH, Lee CM, Kim YS, Yoon SD, Food Res. Int., 100, 377 (2017)
  14. Oh KO, Kim SW, Kim JY, Ko SY, Kim HM, Baek JH, Ryoo HM, Kim JK, Clin. Chim. Acta., 334, 185 (2003)
  15. Yu HH, Seo sJ, Kim YH, Lee HY, Park RK, So HS, Jang SI, You YO, J. Ethnopharmacol., 107, 383 (2006)
  16. Liu CL, Cheng L, Ko CH, Wong CW, Cheng WH, Cheung DWS, Leung PC, Fung KP, Lau CBS, J. Ethnopharmacol., 143, 867 (2012)
  17. Yokozawa T, Kim HY, Tamabe N, Am. J. Chin. Med., 32, 829 (2004)
  18. Son YO, Lee SA, Kim SS, Jang YS, Chun JC, Lee JC, J. Pharm. Pharmacol., 63, 1309 (2011)
  19. Lee H, Kim Y, Kim HJ, Park S, Jang YP, Jung S, Jung H, Bae H, Evid. based. Compl. Alt., 2012, 1 (2012)
  20. Zhang RX, Li MX, Jia ZP, J. Ethnopharmacol., 117, 199 (2008)
  21. Kim HS, Kim KJ, Lee MW, Lee SY, Yun YH, Shim WG, Yoon SD, Int. J. Biol. Macromol., 161, 763 (2020)
  22. Liu XY, Jeshycka S, Lee HJ, Appl. Chem. Eng., 29, 581 (2018)
  23. Zhang MQ, Ren X, Zhao Q, Yue SJ, Fu XM, Li X, Chen KX, Guo YW, Shao CL, Wang CY, J. Ethnopharmacol., 256, 112795 (2020)
  24. Chan EWC, Lim YY, Wong LF, Lianto FS, Wong SK, Lim KK, Joe CE, Lim TY, Food Chem., 109, 477 (2008)
  25. Miliauskas G, Venskutonis PR, Van Beek TA, Food Chem., 85, 231 (2004)
  26. Azmi N, Hashim P, Hashim DM, Halimoon N, Majid NMN, Asian Pac. J. Trop. Med., 4, S348 (2014)
  27. Pillaiyar T, Manickam M, Namasivayam V, J. Enzyme Inhib. Med. Chem., 32, 403 (2017)
  28. Pagning ALN, Tamokou JDD, Lateef M, Tapondjou LA, Kuiate JR, Ngnokam D, Ali MS, Phytochem. Lett., 16, 121 (2016)
  29. Madhan B, Krishnamoorthy G, Rao JR, Nair BU, Int. J. Biol. Macromol., 41, 16 (2007)
  30. Birch-Machin MA, Bowman A, Brit. J. Dermatol., 175, 26 (2016)
  31. Liyanaarachchi GD, Samarasekera JKRR, Mahanama KRR, Hemalal KDP, Ind. Crop. Prod., 111, 597 (2018)
  32. Rafiee SA, Farhoosh R, Sharif A, Eur. J. Lipid. Sci. Tech, 120, 1800319 (2018)
  33. Mishra K, Ojha H, Chaudhury NK, Food Chem., 130, 1036 (2012)
  34. Lee KK, Kim JH, Cho JJ, Choi JD, Int. J. Cosmetic Sci., 21, 71 (1999)