An effective method for cysteine determination based on fluorescence resonance energy system between co-doped graphene quantum dots and silver nanoparticles

Thi Hoa Le; Yong Nam Ahn; Sang Joon Park

Korean Journal of Chemical Engineering, Vol.39, No.4, 1065-1071, April, 2022

DOI10.1007/s11814-021-0956-4 Export Citation

An effective method for cysteine determination based on fluorescence resonance energy system between co-doped graphene quantum dots and silver nanoparticles

Thi Hoa Le, Yong Nam Ahn, and Sang Joon Park^†

Department of Chemical and Biological Engineering, Gachon University, Seongnam 13120, Korea

E-mail:

Cysteine (Cys) is a crucial amino acid. Developing a method for Cys evaluation and detection is necessary for the diagnosis of various diseases. A variety of sensors use graphene quantum dots (GQDs) for biological compound determination; however, GQDs demonstrate very poor fluorescence quantum yield. Therefore, we doped nitrogen and phosphorus into GQDs to form composite material NP-GQDs with enhanced fluorescence properties. NPGQDs were characterized by ultraviolet-visible, fluorescence spectroscopy, transmission electron microscopy, and X-ray photoelectron spectroscopy. Then, we used NP-GQDs as donors and silver nanoparticles (AgNPs) as acceptors to design a fluorescence resonance energy transfer (FRET) system for Cys detection. Optimal conditions for sensing were investigated, and under these conditions our FRET system showed good results in Cys determination. The fluorescence intensity of NP-GQDs was quenched proportionally along with increasing Cys concentration from 0.5 to 4.5 μM and the limit of detection (LOD) was 0.1 μM. In the presence of different amino acids, the FRET system also showed excellent selectivity for the Cys detection.

Keywords:Silver Nanoparticles (AgNPs);Nitrogen-phosphorus Co-doped Graphene Quantum Dots (NP-GQDs);FRET;System;Fluorescence Quenching;Cysteine

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[2] Wang Y, Meng QT, Han Q, He GJ, Hu YY, Feng H, Jia HM, Zhang R, Zhang ZQ, New J. Chem., 42, 15839 (2018)

[3] Qian M, Xia J, Zhang LW, Chen QX, Guo JL, Cui HY, Kafuti YS, Wang JY, Peng XJ, Sens. Actuators B-Chem., 321, 128441 (2020)

[4] Weerapana E, Wang C, Simon GM, Richter F, Khare S, Dillon MBD, Bachovchin DA, Mowen K, Baker D, Cravatt BF, Nature, 468, 790 (2010)

[5] Chung TK, Funk MA, Baker DH, J. Nutr., 120, 158 (1990)

[6] Xie XX, Yin CX, Yue YK, Huo FJ, Sens. Actuators B-Chem., 267, 76 (2018)

[7] Shahrokhian S, Anal. Chem., 73, 5972 (2001)

[8] Yang SL, Li G, Xia N, Wang YX, Liu PP, Qu LB, J. Alloy. Compd., 853, 157077 (2021)

[9] Sahu S, Sharma S, Kant T, Shrivas K, Ghosh KK, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., 246, 118961 (2021)

[10] Hou XF, Li ZS, Li YQ, Zhou QH, Liu CH, Fan D, Wang JJ, Xu RJ, Xu ZH, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., 246, 119030 (2021)

[11] Hong JX, Feng GQ, Sens. Actuators B-Chem., 326, 129016 (2021)

[12] Feng B, Liu Y, Huang S, Huang XY, Huang L, Liu M, Wu JX, Du T, Wang SL, Feng XP, Zeng WB, Sens. Actuators B-Chem., 325, 128786 (2020)

[13] Shi J, Wang Y, Tang X, Liu W, Jiang H, Dou W, Liu W, Dyes Pigment., 100, 255 (2014)

[14] Ming FL, Hou JZ, Hou CJ, Yang M, Wang XF, Li JW, Huo DQ, He Q, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., 222, 8 (2019)

[15] Wu XR, Wu LN, Cao XZ, Li Y, Liu AR, Liu SQ, RSC Adv., 8, 20000 (2018)

[16] Lin LP, Wang YH, Xiao YL, Liu W, Microchim. Acta, 186, 7 (2019)

[17] Xie YT, Zheng JX, Wang YL, Wan JL, Yang YZ, Liu XG, Chen YK, Nanotechnology, 30, 10 (2019)

[18] Le TH, Lee HJ, Kim JH, Park SJ, Sensors, 20, 3470 (2020)

[19] Daniels JK, Chumanov G, J. Electroanal. Chem., 575, 203 (2005)

[20] Jiang HS, Zhang Y, Lu ZW, Lebrun R, Gontero B, Li W, Small, 15, e1900860 (2019)

[21] López-Lorente AI, Soriano ML, Valcárcel M, Microchim. Acta, 181, 1789 (2014)

[22] Gakiya-Teruya M, Palomino-Marcelo L, Rodriguez-Reyes JCF, Methods Protoc., 2, 3 (2019)

[23] Xu Q, Li BF, Ye YC, Cai W, Li WJ, Yang CY, Chen YS, Xu M, Li N, Zheng XS, Street J, Luo Y, Cai LL, Nano Res., 11, 3691 (2018)

[24] Xu Y, Wu M, Liu Y, Feng XZ, Yin XB, He XW, Zhang YK, Chem.-Eur. J., 19, 2276 (2013)

[25] Liu S, Tian JQ, Wang L, Zhang YW, Qin XY, Luo YL, Asiri AM, Al-Youbi AO, Sun XP, Adv. Mater., 24, 2037 (2012)

[26] Amjadi M, Hallaj T, Manzoori JL, Shahbazsaghir T, Spectroc. Acta Pt. A-Molec. Biomolec. Spectr., 201, 223 (2018)

[27] Wang WJ, Peng JW, Li FM, Su BY, Chen X, Chen XM, Microchim. Acta, 186, 32 (2019)

[28] Qu D, Sun ZC, Zheng M, Li J, Zhang YQ, Zhang GQ, Zhao HF, Liu XY, Xie ZG, Adv. Opt. Mater., 3, 360 (2015)

[29] Aryal S, Bahadur KCR, Bhattarai N, Kim CK, Kim HY, J. Colloid Interface Sci., 299, 189 (2006)

[30] Shen ZG, Han GC, Liu CF, Wang XY, Sun RC, J. Alloy. Compd., 686, 82 (2016)

[31] Yola ML, Gupta VK, Eren T, Sen AE, Atar N, Electrochim. Acta, 120, 204 (2014)

[32] Hussain M, Khaliq N, Nisar A, Khan M, Karim S, Khan AA, Yi X, Maqbool M, Ali G, Nanotechnology, 31, 13 (2020)

[33] Yu YT, Wang JB, Xiang H, Ying LK, Wu CY, Zhou HW, Liu HY, Dyes Pigment., 183, 7 (2020)

[34] Yang L, Li G, Xia N, Wang YX, Liu PP, Qu LB, J. Alloy. Compd., 853, 9 (2021)

[35] Zhang YM, Song J, Shao WH, Li J, Microporous Mesoporous Mater., 310, 9 (2021)

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[37] Vladislavic N, Roncevic IS, Buzuk M, Buljac M, Drventic I, J. Solid State Electrochem., 25, 841 (2021)