A label-free approach was employed to selectively detect tetracycline using manganese tungstate (MnWO4) nanowafers and functionalized carbon nanofiber-modified glassy carbon electrode (GCE). MnWO4 nanowafers were synthesized hydrothermally and characterized along with functionalized carbon nanofibers (f-CNFs). Cyclic voltammetry and differential pulse voltammetry were employed to detect tetracycline, and the sensor exhibited linear ranges of detection of 1.75-109.25 µM and 109.25-409.25 µM, respectively. The limit of detection was found to be 0.24 µM (S/ N=3) with a sensitivity of 9.91 µA µM-1 cm-2. Interference studies were carried out with ciprofloxacin (Cip), penicillin (Pen), and amoxicillin (Amox). The modified electrode (MnWO4/f-CNF/GCE) was found to provide sensitive and selective detection of tetracycline in the presence of interfering agents.