Using Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry Analysis

Yong Hyun Lee; So Yeon Park; You Jin Hwang; Jae Kweon Park

Macromolecular Research, Vol.30, No.2, 90-98, February, 2022

DOI10.1007/s13233-022-0013-0 Export Citation

Using Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry Analysis

Yong Hyun Lee, So Yeon Park, You Jin Hwang^{1, †}, and Jae Kweon Park^†

Department of Life Sciences, Gachon University, Seongnamdaero 1342, Seongnam-si, Gyeonggi, 13120, Korea
¹Department of Biomedical Engineering, College of Health Science, Gachon University, 191 Hambakmoe-ro, Yeonsu-gu, Incheon, 21936, Korea

E-mail:,

The antibacterial effect of chitosan with different molecular weights was investigated to define their potent biological activity. Both chitosan hydrolysates designated to CTSN-P30 and CTSN-B8 derived from the hydrolysis of high molecular weight chitosan showed significant synergistic antibacterial effects toward Staphylococcus aureus in the presence of 1,3-butylene glycol. The concentration-dependent antimicrobial effect is very important in determining the minimum inhibitory concentration (MIC) of both CTSN-P30 and CTSN-B8. Each MIC50 of CTSN-P30 and CTSN-B8 was determined to be about 25 and 50 ?g/mL, respectively. Among chitosan hydrolysates, CTSN-P30 can effectively protect the membrane from the penetrating of fine dust which may contain unspecified microorganisms. Also, CTSN-P30, which showed very significant antimicrobial activity, was confirmed to be a hydrolysate containing (GlcNAc)3, and other hydrolysates consisting of (GlcN)5 to (GlcN)15 using matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry analysis. These results demonstrated the feasibility of CTSN-P30 and 1,3-butylene glycol to use as a potent antibacterial and find a dustproof agent for the preparation of various industrial products.

Keywords:antibacterial effect;chitosan;fine dust;1,3-butylene glycol;molecular weight

[References]

Brown CA, Wang B, Oh JH, J. Food Prot., 71, 319 (2008)
Fernandes JC, Tavaria FK, Soares JC, Ramos OS, Monteiro MJ, Pintado ME, Malcata FX, Food Microbiol., 25, 922 (2008)
Kaya M, Asan-Ozusaglam M, Erdogan S, J. Biosci. Bioeng., 121, 678 (2016)
Kinnunen T, Koskela M, Acta. Derm. Venereol., 71, 148 (1991)
Abbas M, Hussain T, Arshad M, Ansari AR, Irshad A, Nisar J, Hussain F, Masood N, Nazir A, Iqbal M, Int. J. Biol. Macromol., 140, 871 (2019)
Alizadeh N, Malakzadeh S, Int. J. Biol. Macromol., 147, 778 (2020)
Radwan-Praglowska J, Piatkowski M, Deineka V, Janus L, Korniienko V, Husak E, Holubnycha V, Liubchak I, Zhurba V, Sierakowska A, Pogorielov M, Bogdal D, Molecules, 24, 2629 (2019)
Szymanska E, Winnicka L, Mar. Drugs, 13, 1819 (2015)
Zhang J, Xia W, Liu P, Cheng Q, Tahirou T, Gu W, Li B, Mar. Drugs, 8, 1962 (2010)
Dong W, Han B, Shao K, Yang Z, Peng Y, Yang Y, Liu W, J. Mater. Sci. Mater. Med., 23, 2945 (2012)
Kumirska J, Weinhold MX, Sauvageau JC, Th?ming J, Kaczy?ski Z, Stepnowski P, J. Pharm. Biomed. Anal., 50, 587 (2009)
Das D, Das N, J. Hazard. Mater., 278, 597 (2014)
Akther T, Ahmed M, Shohel M, Ferdousi FK, Salam A, Environ. Sci. Pollut. Res. Int., 26, 5475 (2019)
Dogra B, Amna S, Park YI, Park JK, Macromol. Res., 25, 172 (2017)
Chae SY, Jang MK, Nah JW, J. Control Release, 102, 383 (2005)
Chen Y, Luo XS, Zhao Z, Chen Q, Wu D, Sun X, Wu L, Jin L, Ecotoxicol. Environ. Saf., 165, 505 (2018)
Soleimani M, Amini N, Sadeghian B, Wang DS, Fang LP, J. Environ. Sci., 72, 166 (2018)
Spence CA, Boyd RD, Wray CD, Whitehead DM, J. Anim. Sci., 60, 1280 (1985)
Lever M, Anal. Biochem., 81, 21 (1977)
Lever M, Walmsley TA, Visser RS, Ryde SJ, Anal. Biochem., 139, 205 (1984)
Mushtaq S, Khan JA, Rabbani F, Latif U, Arfan M, Yameen MA, J. Med. Microbiol., 66, 318 (2017)
Vasile C, Sivertsvik M, Mitelut AC, Brebu MA, Stoleru E, Rosnes JT, Tanase EE, Khan W, Pamfil D, Cornea CP, Irimia A, Popa ME, Materials, 10, 45 (2017)
Yoo AY, Alnaeeli M, Park JK, Process Biochem., 51, 463 (2016)
Lee YH, Park SY, Park JE, Jung BO, Park JE, Park JK, Hwang YJ, Int. J. Mol. Sci., 20, 3085 (2019)
Jirawutthiwongchai J, Klaharn IY, Hobang N, Mai-Ngam K, Klaewsongkram J, Sereemaspun A, Chirachanchai S, Carbohydr. Polym., 141, 41 (2016)
Sakaguchi Y, Watanabe M, Ueoka C, Sugiyama E, Taketomi T, Yamada S, Sugahara K, J. Biochem., 129, 107 (2001)
Vinogradov E, Bock K, Carbohydr. Res., 309, 57 (1998)
Teimouri A, Azami SJ, Keshavarz H, Esmaeili F, Alimi R, Mavi SA, Shojaee S, Int. J. Nanomed., 13, 1341 (2018)
Singh R, Weikert T, Basa S, Moerschbacher BM, Sci. Rep., 9, 1132 (2019)
Seki K, Nishiyama Y, Mitsutomi M, J. Biosci. Bioeng., 127, 425 (2019)
Kashif SA, Park JK, Macromol. Res., 27, 551 (2019)

[1] Brown CA, Wang B, Oh JH, J. Food Prot., 71, 319 (2008)

[2] Fernandes JC, Tavaria FK, Soares JC, Ramos OS, Monteiro MJ, Pintado ME, Malcata FX, Food Microbiol., 25, 922 (2008)

[3] Kaya M, Asan-Ozusaglam M, Erdogan S, J. Biosci. Bioeng., 121, 678 (2016)

[4] Kinnunen T, Koskela M, Acta. Derm. Venereol., 71, 148 (1991)

[5] Abbas M, Hussain T, Arshad M, Ansari AR, Irshad A, Nisar J, Hussain F, Masood N, Nazir A, Iqbal M, Int. J. Biol. Macromol., 140, 871 (2019)

[6] Alizadeh N, Malakzadeh S, Int. J. Biol. Macromol., 147, 778 (2020)

[7] Radwan-Praglowska J, Piatkowski M, Deineka V, Janus L, Korniienko V, Husak E, Holubnycha V, Liubchak I, Zhurba V, Sierakowska A, Pogorielov M, Bogdal D, Molecules, 24, 2629 (2019)

[8] Szymanska E, Winnicka L, Mar. Drugs, 13, 1819 (2015)

[9] Zhang J, Xia W, Liu P, Cheng Q, Tahirou T, Gu W, Li B, Mar. Drugs, 8, 1962 (2010)

[10] Dong W, Han B, Shao K, Yang Z, Peng Y, Yang Y, Liu W, J. Mater. Sci. Mater. Med., 23, 2945 (2012)

[11] Kumirska J, Weinhold MX, Sauvageau JC, Th?ming J, Kaczy?ski Z, Stepnowski P, J. Pharm. Biomed. Anal., 50, 587 (2009)

[12] Das D, Das N, J. Hazard. Mater., 278, 597 (2014)

[13] Akther T, Ahmed M, Shohel M, Ferdousi FK, Salam A, Environ. Sci. Pollut. Res. Int., 26, 5475 (2019)

[14] Dogra B, Amna S, Park YI, Park JK, Macromol. Res., 25, 172 (2017)

[15] Chae SY, Jang MK, Nah JW, J. Control Release, 102, 383 (2005)

[16] Chen Y, Luo XS, Zhao Z, Chen Q, Wu D, Sun X, Wu L, Jin L, Ecotoxicol. Environ. Saf., 165, 505 (2018)

[17] Soleimani M, Amini N, Sadeghian B, Wang DS, Fang LP, J. Environ. Sci., 72, 166 (2018)

[18] Spence CA, Boyd RD, Wray CD, Whitehead DM, J. Anim. Sci., 60, 1280 (1985)

[19] Lever M, Anal. Biochem., 81, 21 (1977)

[20] Lever M, Walmsley TA, Visser RS, Ryde SJ, Anal. Biochem., 139, 205 (1984)

[21] Mushtaq S, Khan JA, Rabbani F, Latif U, Arfan M, Yameen MA, J. Med. Microbiol., 66, 318 (2017)

[22] Vasile C, Sivertsvik M, Mitelut AC, Brebu MA, Stoleru E, Rosnes JT, Tanase EE, Khan W, Pamfil D, Cornea CP, Irimia A, Popa ME, Materials, 10, 45 (2017)

[23] Yoo AY, Alnaeeli M, Park JK, Process Biochem., 51, 463 (2016)

[24] Lee YH, Park SY, Park JE, Jung BO, Park JE, Park JK, Hwang YJ, Int. J. Mol. Sci., 20, 3085 (2019)

[25] Jirawutthiwongchai J, Klaharn IY, Hobang N, Mai-Ngam K, Klaewsongkram J, Sereemaspun A, Chirachanchai S, Carbohydr. Polym., 141, 41 (2016)

[26] Sakaguchi Y, Watanabe M, Ueoka C, Sugiyama E, Taketomi T, Yamada S, Sugahara K, J. Biochem., 129, 107 (2001)

[27] Vinogradov E, Bock K, Carbohydr. Res., 309, 57 (1998)

[28] Teimouri A, Azami SJ, Keshavarz H, Esmaeili F, Alimi R, Mavi SA, Shojaee S, Int. J. Nanomed., 13, 1341 (2018)

[29] Singh R, Weikert T, Basa S, Moerschbacher BM, Sci. Rep., 9, 1132 (2019)

[30] Seki K, Nishiyama Y, Mitsutomi M, J. Biosci. Bioeng., 127, 425 (2019)

[31] Kashif SA, Park JK, Macromol. Res., 27, 551 (2019)