Dynamic Protein Adsorption-Desorption Analysis of Contact Lenses in a Three-Dimensional-Printed Eye Model

Wan-Hsin Chang; Pei-Yi Liu; Dai-En Lin; Yuan-Ting Jiang; Chien-Ju Lu; Yuan-Hao Howard Hsu

Macromolecular Research, Vol.30, No.1, 6-15, January, 2022

DOI10.1007/s13233-022-0003-2 Export Citation

Dynamic Protein Adsorption-Desorption Analysis of Contact Lenses in a Three-Dimensional-Printed Eye Model

Wan-Hsin Chang, Pei-Yi Liu¹, Dai-En Lin¹, Yuan-Ting Jiang¹, Chien-Ju Lu^{1, †}, and Yuan-Hao Howard Hsu^{2, †}

R&D Center, Yung Sheng Optical Co., aya District, Taichung, 42881, Taiwan, Korea
¹R&D Center, Yung Sheng Optical Co., Daya District, Taichung, 42881, Taiwan
²The Department of Chemistry, Tunghai University, Xitun District, Taichung, 40704, Taiwan

E-mail:,

Adsorption of secreted protein on contact lenses is a dynamic and complex behavior. To understand this behavior, we used three-dimensional (3D) printing technology to create an eye model that simulated the anterior segment of the actual human eyeball. In this model, the fluid inlet was connected to a syringe pump to mimic the rate of human tear secretion and the outlet was connected to an ultraviolet-visible (UV-Vis) spectrophotometer. The experimental results revealed that the symmetrical eye model with a 180° inlet-outlet angle was suitable for dynamic analysis of protein adsorption. In this model, protein adsorption was slow and desorption was rapid. The contact lens was soaked in poly(2-methacryloyloxyethyl phosphorylcholine-co-butyl methacrylate) (PMB) to confirm the anti-protein adsorption property of this polymer through dynamic adsorption and desorption eye model analysis.

Keywords:contact lens;three-dimensional printing;eye model;lysozyme;adsorption

[References]

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Patterson K, Lisal M, Colina CM, Fluid Phase Equilib., 302(1-2), 48 (2011)
Bhakta SA, Evans E, Benavidez TE, Garcia CD, Anal Chim Acta, 872, 7 (2015)
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[1] Omali NB, Subbaraman LN, Coles-Brennan C, Fadli Z, Jones LW, Optom Vis Sci., 92, 750 (2015)

[2] Tam NK, Pitt WG, Perez KX, Handly E, Glenn AA, Hickey JW, Larsen BG, Cont Lens Anterior Eye, 37, 405 (2014)

[3] Heynen M, Lorentz H, Srinivasan S, Jones L, Optom Vis Sci, 88, 1172 (2011)

[4] Luensmann D, Jones L, Cont Lens Anterior Eye, 35, 53 (2012)

[5] Zhou L, Zhao SZ, Koh SK, Chen L, Vaz C, Tanavde V, Li XR, Beuerman RW, J Proteomics, 75, 3877 (2012)

[6] Omali NB, Subbaraman LN, Coles-Brennan C, Fadli Z, Jones LW, Optom Vis Sci., 92, 750 (2015)

[7] Luensmann D, Heynen M, Liu L, Sheardown H, Jones L, Mol. Vis., 16, 79 (2010)

[8] Omali NB, Heynen M, Subbaraman LN, Papinski D, Lakkis C, Smith SL, Morgan PB, Berntsen DA, Nichols JJ, Jones LW, Optom. Vis. Sci., 93, 963 (2016)

[9] Ng A, Heynen M, Luensmann D, Subbaraman LN, Jones L, J. Biomed. Mater. Res. Part B Appl. Biomater., 101, 1172 (2013)

[10] Skotnitsky CC, Naduvilath TJ, Sweeney DF, Sankaridurg PR, Optom. Vis. Sci., 83, 27 (2006)

[11] Skotnitsky C, Sankaridurg PR, Sweeney DF, Dsc BAHP, Clin Exp Optom, 85, 193 (2002)

[12] Allansmith MR, Korb DR, Greiner JV, Henriquez AS, Simon MA, Finnemore VM, Am. J. Ophthalmol., 83, 697 (1977)

[13] Solomon A, Curr Opin Allergy Clin Immunol, 16, 492 (2016)

[14] Patterson K, Lisal M, Colina CM, Fluid Phase Equilib., 302(1-2), 48 (2011)

[15] Bhakta SA, Evans E, Benavidez TE, Garcia CD, Anal Chim Acta, 872, 7 (2015)

[16] Burkert S, Bittrich E, Kuntzsch M, Muller M, Eichhorn KJ, Bellmann C, Uhlmann P, Stamm M, Langmuir, 26(3), 1786 (2010)

[17] Vogler EA, Biomaterials, 33, 1201 (2012)

[18] Pitt WG, Perez KX, Tam NK, Handly E, Chinn JA, Liu XM, Maziarz EP, J. Biomed. Mater. Res. Part B Appl. Biomater., 101, 1516 (2013)

[19] Lorentz H, Heynen M, Trieu D, Hagedorn SJ, Jones L, Optom Vis Sci, 89, 856 (2012)

[20] Tam NK, Pitt WG, Perez KX, Hickey JW, Glenn AA, Chinn J, Liu XM, Maziarz EP, Optom Vis Sci, 91 (2014)

[21] Subbaraman LN, Jones L, J. Biomater Sci. Polym. Ed., 21, 343 (2010)

[22] Hall B, Jones JA, Forrest LF, Forrest JA, J. Biomed. Mater. Res A., 101, 755 (2013)

[23] Lee SE, Kim SR, Park M, J. Ophthalmol., 2017, 513176 (2017)

[24] Scheuer CA, Fridman KM, Barniak VL, Burke SE, Venkatesh S, Cont Lens Anterior Eye, 33, S2 (2010)

[25] Bajgrowicz M, Phan CM, Subbaraman LN, Jones L, Investig. Ophthalmol. Vis. Sci., 56, 2234 (2015)

[26] Yao H, Liao YT, Lingley A, Afanasiev A, Lahdesmaki I, Otis B, Parviz B, J. Micromech. Microeng., 22 (2012)

[27] Yao H, Afanasiev A, Lahdesmaki I, Parviz BA, in 2011 IEEE 24th International Conference on Micro Electro Mechanical Systems pp.25, 2011.

[28] Deng X, Korogiannaki M, Rastegari B, Zhang J, Chen M, Fu Q, Sheardown H, Filipe CDM, Hoare T, ACS Appl. Mater. Interfaces, 8, 22064 (2016)

[29] Nishigochi S, Ishigami T, Maruyama T, Hao Y, Ohmukai Y, Iwasaki Y, Matsuyama H, Ind. Eng. Chem. Res., 53(6), 2491 (2014)

[30] Zhang Y, Yi M, Bao Y, Zhang S, Polym Int, 67, 155 (2018)

[31] Zhang N, Chen C, Selo MAS, Bai YX, Cheng L, Xu HHK, Int. J. Oral. Sci., 7, 103 (2015)

[32] Vales TP, Jee JP, Lee WY, Cho S, Lee GM, Kim HJ, Kim JS, Materials, 13 (2020)

[33] Ishihara K, Mu M, Konno T, Inoue Y, Fukazawa K, J. Biomater. Sci. Polym. Ed., 28, 884 (2017)

[34] Lee I, Kobayashi K, Sun HY, Takatani S, Zhong LG, J. Biomed. Mater. Res. A, 82, 316 (2017)

[35] Ishihara K, Iwasaki Y, Nakabayashi N, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 6, 253 (1998)

[36] Elliott AM, et al., 23rd Annual International Solid Freeform Fabrication Symposium - An Additive Manufacturing Conference, SFF 2012, 988 (2012).

[37] Xu X, Robles-Martinez P, Madla CM, Joubert F, Goyanes A, Basit AW, Gaisford S, Addit Manuf, 33, 101071 (2020)

[38] Kashte S, Jaiswal AK, Kadam S, Tissue Eng Regen Med, 14, 1 (2017)

[39] Trombetta R, Inzana JA, Schwarz EM, Kates SL, Awad HA, Ann Biomed Eng, 45, 23 (2017)

[40] Blaeser A, Campos DFD, Puster U, Richtering W, Stevens MM, Fischer H, Adv. Healthc. Mater., 5, 326 (2016)

[41] Jariwala SH, Lewis GS, Bushman ZJ, Adair JH, Donahue HJ, 3D Print Addit Manuf, 2, 56 (2015)

[42] Bao X, Zhu L, Huang X, Tang D, He D, Shi J, Xu G, Sci. Rep., 7, 7814 (2017)

[43] Huang X, Lin S, Hu Y, Liao Y, Wang W, Zhang Z, Rui G, Cai P, J Biomater Tissue Eng, 8, 1038 (2018)

[44] Goodacre BJ, Swamidass RS, Lozada J, Al-Ardah A, Sahl E, J Prosthet Dent, 119, 897 (2018)

[45] Schweiger J, Beuer F, Stimmelmayr M, Edelhoff D, Magne P, Guth JF, British Dental Journal, 221, 555 (2016)

[46] Reymus M, Fabritius R, Keßler A, Hickel R, Edelhoff D, Stawarczyk B, Clin Oral Investig, 24, 701 (2020)

[47] Goyanes A, Martinez PR, Buanz A, Basit AW, Gaisford S, Int. J. Pharm., 494, 657 (2015)

[48] Hollander J, Genina N, Jukarainen H, Khajeheian M, Rosling A, Makila E, Sandler N, J. Pharm. Sci., 105, 2665 (2016)

[49] Bron AJ, Tiffany JM, Gouveia SM, Yokoi N, Voon LW, Exp. Eye Res., 78, 347 (2004)

[50] Chang WH, Liu PY, Lu CJ, Lin DE, Lin MH, Jiang YT, Hsu YHH, Macromol Res, (2020).

[51] Leahy CD, Mandell RB, Lin ST, Optom Vis Sci., 67, 504 (1990)

[52] Mochizuki H, Yamada M, Hatou S, Kawashima M, Hata S, Eye & Contact Lens, 34 (2008).

[53] Goda T, Ishihara K, Miyahara Y, J. Appl. Polym. Sci., 132 (2015)

[54] Iwasaki Y, Ishihara K, Anal. Bioanal. Chem., 381, 534 (2005)