In present study, sodium carbonate (Na2CO3) and ammonium carbonate ((NH4)2CO3) are separately selected as precipitant to synthesize the strontium oxide (SrO) based solid catalyst, namely, SrO-M/Na and SrO-M/NH4 for biodiesel production through transesterification. The influence of precipitant on catalyst physicochemical property, catalytic activity as well as reusability are investigated and compared. To begin with, transesterification parameters of catalyst amount, molar ratio of methanol to oil, reaction time, and reaction temperature are concerned to comprehensively evaluate catalytic performance of the two catalysts. Moreover, catalyst reusability is evaluated under the optimal conditions. With catalyst amount of 4 wt.%, molar ratio of methanol to oil of 9 at 65 celcius within 30 min, SrO-M/Na exhibits a better catalytic activity in comparison to SrO-M/NH4, whereas, the reusability is in reverse sequence. In order to reveal the reason behind, catalysts are characterized by thermogravimetric/differential thermogravimetric/scanning calorimetry (TG/DTG/DSC), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), N2-adsorption/desorption, basic strength, basicity and vibrating sample magnetometer (VSM).