This review paper proposes a critical overview of experimental techniques and innovative experimental methods to observe and deeply understand the migration of water inside wood and biosourced materials. The state of the art of the knowledge of water transfer phenomena are first presented, namely liquid and bound water migration, together with shrinkage/swelling. Then, the papers presenting the 3D imaging techniques at high resolution offered by recent technologies, such as magnetic resonance imaging, X-ray tomography, and in situ tomography-mechanical tests. They enable visualization and analysis of water transfer mechanisms in eco-responsible materials such as natural fiber-concretes, wood, and biobased insulating materials. The 2D and 3D images processed with specific software packages allow for an overview of the distribution and orientation of the material components, as well as the moisture-content field which affects most properties of the materials such as mechanical properties (compression and tensile strength), hygro-thermal performance, as well as durability. Finally, the Digital Volume Correlation method allows for the observation and evaluation of deformation, cracks and failure mechanisms resulting from either a mechanical or hydric loading. The paper intends to help the reader to acquire a deep understanding of water imbibition and drying mechanisms in such materials.