In the present study, the zero valent zinc (ZVZ) was used in the field of tissue regeneration engineering by inducing the preadipocytes into mature adipocytes in mouse 3T3-L1 cells. Initially, the ZVZ nanoparticles was synthesized using chemical reduction method and confirmed by EDS spectrum analysis which showed the presence of zinc ion without any impurities. The XRD and Raman analysis showed the characteristic peaks of zinc with hexagonal structure and the strong phonon band attributed to ZVZ, respectively. The microscopic analysis showed the ZVZ nanoparticles are well dispersed with spherical shape and the size was found to between 85-95 nm. The in vitro cytotoxic activity of ZVZ against 3T3-L1 showed less toxicity in higher concentration and the results were compared with standard adipogenic stimulant dexamethasone. The oil red O staining assay morphologically confirmed the ZVZ induces adipogenesis in 3T3-L1 as well as increase the lipid accumulation during treatment with ZVZ. Moreover, the ZVZ upregulates the expression of adipogenic marker genes such as PPAR gamma, FABP4 and the calpain family protein during differentiation of preadipocytes into mature adipocytes. Together, the ZVZ regulates the differentiation in 3T3-L1 cells through PPAR gamma and calpain signaling pathway deciphers the role of ZVZ in tissue regeneration engineering.