The giant unilamellar vesicle (GUV) is a basic model of the cell membrane that allows for the modulation and control of membrane shape dynamics, which play essential roles in the functions of living cell membranes. However, to properly use these artificial cell-like model systems, we need to understand their physical properties. GUV generation techniques are key technologies in the synthesis of artificial cell-like model systems. However, it is unclear whether GUVs produced by different techniques have the same physical properties. Here, we have investigated the physical properties of GUVs prepared by inverted emulsion and hydration techniques by examining the membrane shape deformation induced by external stimulation with a nonionic surfactant. We reveal differences in the spontaneous curvature of the membrane, the preferred differential area between the inner and outer leaflets of the membrane, and the edge tension of membrane pores between the GUVs prepared using the two distinct techniques.