The present work reports the synthesis of the novel flower-shaped Pt-Cu Janus nanoparticles (JNPs) with the sonochernical approach as a heterogeneous catalyst for the selective oxidation of glycerol. The nucleation and growth of Pt on Cu were attained with the seed-mediated route. The present approach delivers better control over the morphology of JNPs with respect to the conventional coreduction method. The images obtained from high-resolution transmission electron microscopy showed the flower-shaped structure of the synthesized JNPs. The catalytic activity of the synthesized Janus Pt-Cu/C nanocatalyst was studied for the glycerol oxidation reaction. The glycerol conversion of 96% and selectivity of 65% toward glyceric acid were obtained within a reaction time of 1 h by using as-prepared JNPs as the catalyst. Further, after a reaction time of 4 h, 98.8% glycerol conversion with an excellent combined selectivity of 74% for dicarboxylic acids was achieved. This shows the exceptional catalytic performance of the synthesized Pt-Cu/C JNPs for the selective glycerol oxidation with mild operating conditions.