화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Macromolecular Research, Vol.29, No.3, 191-198, March, 2021
DOI10.1007/s13233-021-9028-1  Export Citation
Coating of Laponite on PLA Nanofibrous for Bone Tissue Engineering Application
Zahra Orafa1, Shiva Irani1, †, Ali Zamanian2, Hadi Bakhshi3, Habib Nikukar4, 5, and Behafarid Ghalandari6
  • 1Department of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran
  • 2Biomaterials Research Group, Department of Nanotechnology and Advanced Materials, Materials and Energy Research Center, Tehran, Iran
  • 3Department of Functional Polymer Systems, Fraunhofer Institute for Applied Polymer Research IAP, Geiselbergstraße 68, 14476 Potsdam, Germany
  • 4Medical Nanotechnology and Tissue Engineering Research Center, Yazd Reproductive Sciences Institute, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • 5Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran
  • 6Department of Medical Nanotechnology, Applied Biophotonics Research Center, Science and Research Branch, Islamic Azad University, Tehran, Iran
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In this study, electrospun polylactic acid (PLA) nanofibers were coated with osteoinductive Laponite (LAP) nanoplatelets and used as scaffolds for bone tissue engineering. The LAP nanoplatelets were physically crosslinked with high molecular weight polyethylene oxide (PEO) to make a layer of LAP/PEO network around PLA nanofibers with a thickness of 45-175 nm that was controllable through adjusting the concentration of the LAP/PEO solution (0.4, 0.8, and 1.2 wt%). The LAP/PEO layer improved the surface hydrophilicity (water contact angle from 111° to 26°) and thus the cell attachment and proliferation (p ≤ 0.05) by the scaffolds. The LAP nanoplatelets coated on the surface of PLA nanofibers could induce osteodifferentiation on human mesenchymal stem cells (hMSCs) by increasing the ALKALINE PHOSPHATASE (ALP) activity. The expression of specific osteogenic genes (ALP and OSTEONECTIN) at the transcription level (p ≤ 0.001) for the seeded hMSCs proved the osteoinductive effect of coated LAP nanoplatelets for the differentiation of stem cells to osteoblasts without using any external osteogenic inducers. The LAP-coated nanofibers can be used as an excellent scaffold for bone tissue engineering to provide an appropriate environment for direct bone tissue engineering.
Keywords:polylactic acid nanofibers;Laponite;osteodifferentiation;bone tissue engineering
  1. Zhai X, Ma Y, Hou C, Gao F, Zhang Y Ruan C, Pan H, Lu WW, Liu W, ACS Biomater. Sci. Eng., 3, 1109 (2017)
  2. Wang C, Wang S, Li K, Ju Y, Li J, Zhang Y, Li J, Liu X, Shi X, Zhao Q, PLoS One, 9, e99585 (2014)
  3. Yao Q, Cosme JGL, Xu T, Miszuk JM, Picciani PHS, Fong H, Sun H, Biomaterials, 115, 115 (2017)
  4. Sharifi F, Atyabi SM, Irani S, Bakhshi H, Carbohydr. Polym., 231, 115681 (2020)
  5. Shapourzadeh A, Atyabi SM, Irani S, Bakhshi H, Int. J. Biol. Macromol., 150, 152 (2020)
  6. Alemi PS, Atyabi SA, Sharifi F, Mohamadali M, Irani S, Bakhshi H, Atyabi SM, Polym. Adv. Technol., 30, 1356 (2019)
  7. Esmailian S, Irani S, Bakhshi H, Zandi M, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 92, 800 (2018)
  8. Dabouian A, Bakhshi H, Irani S, Pezeshki-Modaress M, RSC Adv., 8, 9941 (2018)
  9. Tao L, Zhonglong L, Ming X, Zezheng Y, Zhiyuan L, Xiaojun Z, Jinwu W, RSC Adv., 7, 54100 (2017)
  10. Bhaskar B, Owen R, Bahmaee H, Wally Z, Rao PS, Reilly GC, J. Biomed. Mater. Res. Part A, 106, 1334 (2018)
  11. Abdallah O, Jalali F, Zamani S, Isamil HM, Ma S, Nasrallah GK, Younes HM, Pharm. Nanotechnol., 4, 191 (2016)
  12. Arab-Ahmadi S, Irani S, Bakhshi H, Atyabi F, Ghalandari B, Polym. Adv. Technol., 32, 755 (2021)
  13. Sharifi F, Atyabi SM, Norouzian D, Zandi M, Irani S, Bakhshi H, Int. J. Biol. Macromol., 115, 243 (2018)
  14. Fattahi FS, Khoddami A, Avinc O, J. Textiles Polym., 7, 47 (2019)
  15. Habel C, Schottle M, Daab M, Eichstaedt NJ, Wagner D, Bakhshi H, Agarwal S, Horn MA, Breu J, Macromol. Mater. Eng., 303, 180033 (2018)
  16. Castro-Aguirre E, Auras R, Selke S, Rubino M, Marsh T, Polymers, 10, 202 (2018)
  17. Li T, Liu ZL, Xiao M, Yang ZZ, Peng MZ, Li CD, Zhou XJ, Wang JW, Stem Cell. Res. Ther., 9, 100 (2018)
  18. Atrian M, Kharaziha M, Emadi R, Alihosseini F, Appl. Clay Sci., 174, 90 (2019)
  19. Tomas H, Alves CS, Rodrigues J, Nanomed. Nanotechnol. Biol. Med., 14, 2407 (2018)
  20. Becher TB, Braga CB, Bertuzzi DL, Ramos MD, Hassan A, Crespilho FN, Ornelas C, Soft Matter, 15, 1278 (2019)
  21. Wang G, Maciel D, Wu Y, Rodrigues J, Shi X, Yuan Y, Liu C, Tomas H, Li Y, ACS Appl. Mater. Interfaces, 6, 16687 (2014)
  22. Gaharwar AK, Kishore V, Rivera C, Bullock W, Wu CJ, Akkus O, Schmidt G, Macromol. Biosci., 12, 779 (2012)
  23. Wang S, Castro R, An X, Song C, Luo Y, Shen M, Tomas H, Zhu M, Shi X, J. Mater. Chem., 22, 23357 (2012)
  24. Wang Y, Cui W, Chou J, Wen S, Sun Y, Zhang H, Colloids Surf. B: Biointerfaces, 172, 90 (2018)
  25. Gaharwar AK, Rivera CP, Wu CJ, Schmidt G, Acta Biomater., 7, 4139 (2011)
  26. Agrawal SK, Sanabria-DeLong N, Tew GN, Bhatia SR, Langmuir, 24(22), 13148 (2008)
  27. Mahdavinia GR, Ettehadi S, Amini M, Sabzi M, RSC Adv., 5, 44516 (2015)
  28. Palkova H, Madejova J, Zimowska M, Serwicka EM, Microporous Mesoporous Mater., 127, 237 (2010)
  29. Anilkumar KM, Jinisha B, Manoj M, Jayalekshmi S, Eur. Polym. J., 89, 249 (2017)
  30. Khakestani M, Jafari SH, Zahedi P, Bagheri R, Hajiaghaee R, J. Appl. Polym. Sci., 134, 45343 (2017)
  31. Xu W, Shen R, Yan Y, Gao J, J. Mech. Behav. Biomed. Mater., 65, 428 (2017)
  32. Loiola LMD, Mas BA, Duek EAR, Felisberti MI, Eur. Polym. J., 68, 618 (2015)
  33. Li HL, Zhou GX, Shan YK, Yuan ML, in Advanced Materials Research, Trans Tech Publ, Vol. 706, pp 340-343 2013.
  34. Shao W, He J, Sang F, Wang Q, Chen L, Cui S, Ding B, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 62, 823 (2016)
  35. Tang L, Wei W, Wang X, Qian J, Li J, He A, Yang L, Jiang X, Li X, Wei J, RSC Adv., 8, 10794 (2018)
  36. Kerativitayanan P, Tatullo M, Khariton M, Joshi P, Perniconi B, Gaharwar AK, ACS Biomater. Sci. Eng., 3, 590 (2017)
  37. Gaharwar AK, Schexnailder PJ, Kline BP, Schmidt G, Acta Biomater., 7, 568 (2011)
  38. Gaharwar AK, Mihaila SM, Swami A, Patel A, Sant S, Reis RL, Marques AP, Gomes ME, Khademhosseini A, Adv. Mater., 25(24), 3329 (2013)
  39. George KS, Munoz J, Akhavan NS, Foley EM, Siebert SC, Tenenbaum G, Khalil DA, Chai SC, Arjmandi BH, Food Funct., 11, 544 (2020)
  40. Mihaila SM, Gaharwar AK, Reis RL, Khademhosseini A, Marques AP, Gomes ME, Biomaterials, 35, 9087 (2014)
  41. Prabha RD, Nair BP, Ditzel N, Kjems J, Nair PD, Kassema M, Mater. Sci. Eng. C-Biomimetic Supramol. Syst., 94, 509 (2019)
  42. Guweidhi A, Kleeff J, Adwan H, Giese NA, Wente MN, Giese T, Buchler MW, Berger MR, Friess H, Ann. Surg., 242, 224 (2005)