The objective of this study was to assess the role of UbK, a novel protein kinase, in the growth ofBacillus subtilis, especially under oxidative stress conditions. Growth profiles of wild-type and Delta ubKmutant strains were assessed in the presence of paraquat, an in vivo inducer of oxidative stress. Wild-typeB. subtiliscells were able to efficiently survive the stress conditions, whereas the growth profile of the Delta ubKmutant strain was significantly affected. Complementation of the Delta ubKmutant with a plasmid coding for a wild-type UbK restored wild-type growth phenotypes. Furthermore, we used recombinant plasmids containing the genes of the active kinase (UbK) and its inactive form (E106AUbK) to transform wild-type and Delta ubKmutant strains. Our results showed that an active form of UbK is needed to restore a normal growth profile. Protein kinases allow a fine-tuning of cellular processes, including those related to metabolic adaptation to environmental cues. Our findings highlight the importance of an active UbK in the bacterial growth under oxidative stress inB. subtilis. This study revealed the role of a new protein kinase, UbK, allowingB. subtilisto survive oxidative stress.