Strain HX-22-1(T)was isolated from the sludge collected from the outlet of the biochemical treatment facility of an agricultural chemical factory in Nanjing city, Jiangsu province, PR China. Strain HX-22-1(T)is Gram-staining-negative, rod-shaped, non-spore-forming and non-flagellated. The 16S rRNA gene-based phylogenetic analysis indicates that the strain HX-22-1(T)belongs to the genusPedobacter, closely related toPedobacter glucosidilyticusKCTC 22438(T)(98.63% similarity). The genomic DNA G+C content of the strain was 34.4 mol%. Strain HX-22-1(T)was able to grow at 16-37 degrees C (optimum at 30 degrees C), at pH 6.0-8.0 (optimum at pH 7.0), and with 0-1% (w/v) NaCl (optimum at 0). Predominant fatty acid constituents were iso-C(15:0)and summed feature 3 (iso-C-16:1 omega 7cand/or C-16:1 omega 6c). The predominant respiratory ubiquinone was MK-7. The polar lipid profile is composed of phosphatidylethanolamine (PE), aminophospholipid (APL), aminolipid (AL), and phospholipids (PL). The ANI and dDDH values obtained between the genomes of HX-22-1(T)andP.glucosidilyticusKCTC 22438(T)were 89.6 and 38.8%, respectively. On the basis of data from phenotypic, chemotaxonomic, and genotypic analysis, strain HX-22-1(T)represents a novel species of the genusPedobacter, for which the namePedobacter puniceumsp. nov. is proposed. The type strain is HX-22-1(T)(= KCTC 72655(T) = CCTCC AB 2019348(T)).